Mouse CD99 / MIC2 Monoclonal Antibody | anti-CD99 antibody

CD99 / MIC2 (Ewing's Sarcoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439059

• Gene Names: CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• Reactivity: Human, Rat.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: CD99 / MIC2; Monoclonal Antibody; CD99 / MIC2 (Ewing's Sarcoma Marker) Mouse Monoclonal Antibody; 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2; anti-CD99 antibody

• Host: Mouse
• Reactivity: Human, Rat.; Others not tested
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: HO36-1.1
• Specificity: Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 169

• Applicable Applications for anti-CD99 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (5-10ul/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at Room Temp) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cell Surface
• Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes
• Hu-Chromosome Location: Xp22.33
• Positive Control: Pancreas or Ewing's sarcoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (HO36-1.1).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with CD99 Monoclonal Antibody (HO36-1.1).)

References

Sandrin MS, et. al. Immunogenetics, 1992, 35(4):283-5

NCBI and Uniprot Product Information

• NCBI GI #: 171543879
• NCBI GeneID: 4267
• NCBI Accession #: NP_001116370.1
• NCBI GenBank Nucleotide #: NM_001122898.2
• UniProt Accession #: P14209; O00518; Q6ICV7; A6NIW1
• Molecular Weight: 27-32kDa
• NCBI Official Full Name: CD99 antigen isoform b
• NCBI Official Synonym Full Names: CD99 molecule
• NCBI Official Symbol: CD99
• NCBI Official Synonym Symbols: MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• NCBI Protein Information: CD99 antigen
• UniProt Protein Name: CD99 antigen
• Protein Family: CD99 antigen
• UniProt Gene Name: CD99
• UniProt Synonym Gene Names: MIC2; MIC2X; MIC2Y
• UniProt Entry Name: CD99_HUMAN

NCBI Description

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]

Uniprot Description

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: cytoplasm; focal adhesion; integral to plasma membrane; plasma membrane