IL-29 elisa kit
Human IL-29 ELISA Kit
Reactivity
No cross reactivity observed with IFNa2, IL-28A, IL-28B, IFNg, IL-10, IL-24, IP-10, IL-31, IL-34 and GM-CSF
Synonyms
IL-29; Human IL-29 ELISA Kit; IL-29 elisa kit
Reactivity
No cross reactivity observed with IFNa2, IL-28A, IL-28B, IFNg, IL-10, IL-24, IP-10, IL-31, IL-34 and GM-CSF
Specificity
The assay recognizes natural and recombinant human IL-29. To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IFN2, IL28A, IL28B, IFN, IL-10, IL-24, IP-10, IL-31, IL-34 and GM-CSF).
Samples
Cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids
Assay Type
Sandwich
Sensitivity
5.9 pg/ml
Intra-assay Precision
Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in culture media and 2 in standard diluent with samples containing different concentrations of IL-29. The overall intra-assay coefficient of variation has been calculated to be 5.0%.
Inter-assay Precision
Assay to assay reproducibility within one laboratory was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in culture media and 2 in standard diluent with samples containing different concentrations of IL-29. The calculated overall coefficient of variation was 9.9%.
Related Product Information for IL-29 elisa kit
Intended Uses: The human IL-29 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of IL-29 in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human IL-29.
Principle of the Assay: The IL-29 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL-29 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-29 concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-29 antigen and a biotinylated monoclonal antibody specific for IL-29 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxidase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-29 present in the samples.
Principle of the Assay: The IL-29 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL-29 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-29 concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-29 antigen and a biotinylated monoclonal antibody specific for IL-29 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxidase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-29 present in the samples.
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Product Notes
The No cross reactivity observed with IFNa2, IL-28A, IL-28B, IFNg, IL-10, IL-24, IP-10, IL-31, IL-34 and GM-CSF IL-29 (Catalog #AAA335765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA335765 ELISA Kit recognizes No cross reactivity observed with IFNa2, IL-28A, IL-28B, IFNg, IL-10, IL-24, IP-10, IL-31, IL-34 and GM-CSF IL-29. It is sometimes possible for the material contained within the vial of "IL-29, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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