Typical Testing Data/Standard Curve (for reference only)
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IL-2 elisa kit
Human IL-2 HS ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: The IL-2 high sensitivity ELISA Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-2 antigen and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IL-2 present in the samples.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Uniprot Description
IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.
Protein type: Oncoprotein; Secreted, signal peptide; Cytokine; Secreted
Chromosomal Location of Human Ortholog: 4q26-q27
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; interleukin-2 receptor binding; cytokine activity; kinase activator activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding
Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; positive regulation of inflammatory response; negative regulation of apoptosis
Research Articles on IL-2
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Product Notes
The Human. No cross reactivity with other human cytokines. IL-2 il-2 (Catalog #AAA335490) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA335490 ELISA Kit recognizes Human. No cross reactivity with other human cytokines. IL-2. It is sometimes possible for the material contained within the vial of "IL-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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