Typical Testing Data/Standard Curve (for reference only)
Rat ICAM-1 / CD54 ELISA Kit | ICAM-1 / CD54 elisa kit
ICAM-1 / CD54 Rat ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ICAM-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ICAM-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ICAM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ICAM-1 can be calculated.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Ref.12 Ref.13 Ref.14 Ref.18
Subunit structure: Homodimer
Probable. Interacts with human herpesvirus 8 MIR2 protein
Probable. Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus. Ref.14 Ref.15 Ref.18 Ref.28 Ref.29
Subcellular location: Membrane; Single-pass type I membrane protein.
Post-translational modification: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Polymorphism: Homozygotes with ICAM1-Kalifi Met-56 seem to have an increased risk for cerebral malaria [
MIM:611162].
Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Research Articles on ICAM-1 / CD54
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Product Notes
The Rat ICAM-1 / CD54 icam1 (Catalog #AAA355378) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355378 ELISA Kit recognizes Rat ICAM-1 / CD54. AAA Biotech's ICAM-1 / CD54 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of ICAM-1 in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the ICAM-1 / CD54 icam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAM-1 / CD54, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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