Plasminogen Activator, Urokinase Receptor (uPAR) Recombinant Protein | uPAR recombinant protein

Recombinant Plasminogen Activator, Urokinase Receptor (uPAR)

Cat. Num. AAA2009582

• Gene Names: Plaur; Cd87; uPAR; u-PAR
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Plasminogen Activator; Urokinase Receptor (uPAR); Recombinant Plasminogen Activator; uPAR recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal GST-his-Tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -TCVPASWGLR CMQCKTNGDC RVEECALGQD LCRTTIVRLW EEGEELELVE KSCTHSEKTN RTLSYRTGLK ITSLTEVVCG LDLCNQGNSG RAVTYSRSRY LECISCGSSD MSCERGRHQS LQCRSPEEQC LDVVTHWIQE GEEGRPKDDR HLRGCGYLPG CPGSNGFHNN DTFHFLKCCN TTKCNEGPIL ELENLPQN
• Sequence Length: 327

• Applicable Applications for uPAR recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Thr15~Asn211 (Accession # P35456) with N-terminal GST-his-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7242179
• NCBI GeneID: 18793
• NCBI Accession #: NP_035243.1
• NCBI GenBank Nucleotide #: NM_011113.3
• UniProt Accession #: P35456; P35457
• Molecular Weight: 52kDa
• NCBI Official Full Name: urokinase plasminogen activator surface receptor
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: Plaur
• NCBI Official Synonym Symbols: Cd87; uPAR; u-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor; urokinase plasminogen activator receptor; urokinase-type plasminogen activator receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: Plaur
• UniProt Synonym Gene Names: U-PAR; uPAR
• UniProt Entry Name: UPAR_MOUSE

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein domain specific binding; protein binding; enzyme binding; U-plasminogen activator receptor activity; receptor binding

Biological Process: mesenchymal cell differentiation; negative regulation of apoptosis