Human ICAM-1 / CD54 ELISA Kit | ICAM-1 / CD54 elisa kit

ICAM-1 / CD54 Human ELISA Kit

Cat. Num. AAA355304

• Gene Names: ICAM1; BB2; CD54; P3.58
• Reactivity: Human
• Applications: ELISA
• Synonyms: ICAM-1 / CD54; ICAM-1 / CD54 Human ELISA Kit; Human ICAM-1 / CD54; ICAM-1 / CD54 elisa kit

• Reactivity: Human

• Applicable Applications for ICAM-1 / CD54 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of ICAM-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ICAM-1 / CD54 elisa kit

Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ICAM-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ICAM-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ICAM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ICAM-1 can be calculated.

Background: ICAM-1 (Intercellular Adhesion Molecule 1) also known as CD54 (Cluster of Differentiation 54) is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor. ICAM-1 is a member of the immunoglobulin superfamily, it can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) and is expressed by the vascular endothelium, macrophages, and lymphocytes.It is also an endothelial- and leukocyte-associated transmembrane protein long known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus.

NCBI and Uniprot Product Information

• NCBI GI #: 167466198
• NCBI GeneID: 3383
• NCBI Accession #: NP_000192.2
• NCBI GenBank Nucleotide #: NM_000201.2
• UniProt Accession #: P05362; Q5NKV7; Q96B50; B2R6M3
• Molecular Weight: 57,825 Da
• NCBI Official Full Name: intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: ICAM1
• NCBI Official Synonym Symbols: BB2; CD54; P3.58
• NCBI Protein Information: intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• UniProt Protein Name: Intercellular adhesion molecule 1
• UniProt Gene Name: ICAM1
• UniProt Synonym Gene Names: ICAM-1
• UniProt Entry Name: ICAM1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Ref.12 Ref.13 Ref.14 Ref.18

Subunit structure: Homodimer

Probable. Interacts with human herpesvirus 8 MIR2 protein

Probable. Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus. Ref.14 Ref.15 Ref.18 Ref.28 Ref.29

Subcellular location: Membrane; Single-pass type I membrane protein.

Post-translational modification: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Polymorphism: Homozygotes with ICAM1-Kalifi Met-56 seem to have an increased risk for cerebral malaria [

MIM:611162].

Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.