Sheep GPX3 ELISA Kit | GPX3 elisa kit

Sheep GPX3 (Glutathione Peroxidase 3, Plasma) ELISA Kit

Cat. Num. AAA2503253

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Sheep
• Synonyms: GPX3; Sheep GPX3 (Glutathione Peroxidase 3; Plasma) ELISA Kit; GPX3 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinantSheepGPX3. No significant cross-reactivity or interference between SheepGPX3 and analogues was observed.
• Sequence Length: 226

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPX3 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of SheepGPX3concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GPX3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPX3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPX3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GPX3.You can calculate the concentration of GPX3in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075.2
• NCBI GenBank Nucleotide #: NM_002084.3
• UniProt Accession #: P22352; O43787; Q86W78; Q9NZ74; Q9UEL1
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase 3
• NCBI Official Synonym Full Names: glutathione peroxidase 3 (plasma)
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3; GPx-3; extracellular glutathione peroxidase; plasma glutathione peroxidase
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Secreted; Other Amino Acids Metabolism - glutathione; Oxidoreductase; EC 1.11.1.9; Secreted, signal peptide; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding

Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; response to lipid hydroperoxide; protein homotetramerization