Mouse anti-Human Glutathione Peroxidase 3 Monoclonal Antibody | anti-GPX3 antibody

Glutathione Peroxidase 3 Antibody (Clone 55A)

Cat. Num. AAA840072

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Ammonium sulphate precipitation
• Synonyms: Glutathione Peroxidase 3; Monoclonal Antibody; Glutathione Peroxidase 3 Antibody (Clone 55A); GPX3; anti-GPX3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 55A
• Purity/Purification: Ammonium sulphate precipitation
• Form/Format: 100 ul of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol; Appearance: Colorless liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 226

• Applicable Applications for anti-GPX3 antibody: Immunoprecipitation (IP), ELISA (EIA), Western Blot (WB)
• Application Notes: IP: 1-2 ul, ELISA.
• Immunogen: Recombinant human protein purified from E.coli
• Positive Control: WB and IP:Bosc23 cell lysate
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-GPX3 antibody

Monoclonal antibody to detect Glutathione Peroxidase 3 in human samples.

Background: Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic /mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

Product Categories/Family for anti-GPX3 antibody

Signal Transduction; Signal Transduction Antibodies (A-Z) Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075.2
• NCBI GenBank Nucleotide #: NM_002084.3
• UniProt Accession #: P22352; O43787; Q86W78; Q9NZ74; Q9UEL1
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase 3
• NCBI Official Synonym Full Names: glutathione peroxidase 3
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Lipid Metabolism - arachidonic acid; Secreted; EC 1.11.1.9; Other Amino Acids Metabolism - glutathione; Secreted, signal peptide; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding

Biological Process: response to reactive oxygen species; response to lipid hydroperoxide; hydrogen peroxide catabolic process; protein homotetramerization