Typical Testing Data/Standard Curve (for reference only)
Canine CD30 Ligand ELISA Kit | CD30L elisa kit
Canine CD30 Ligand ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This CD30L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CD30L. This ELISA kit for research use only! br>
PRINCIPLE OF THE ASSAY CD30L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD30L antibody and an CD30L-HRP conjugate. The assay sample and buffer are incubated together with CD30L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD30L concentration since CD30L from samples and CD30L-HRP conjugate compete for the anti-CD30L antibody binding site. Since the number of sites is limited, as more sites are occupied by CD30L from the sample, fewer sites are left to bind CD30L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD30L concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
TNFSF8: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Belongs to the tumor necrosis factor family.
Protein type: Cytokine; Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 9q33
Cellular Component: extracellular space; integral to plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: cell proliferation; defense response to Gram-positive bacterium; CD8-positive, alpha-beta T cell differentiation; cell-cell signaling; apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; signal transduction
Research Articles on CD30L
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Product Notes
The Canine CD30L tnfsf8 (Catalog #AAA737774) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737774 ELISA Kit recognizes Canine CD30L. It is sometimes possible for the material contained within the vial of "CD30 Ligand, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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