Human Caspase Activated DNase (CAD) ELISA Kit | CAD elisa kit

Human Caspase Activated DNase (CAD) ELISA Kit

Cat. Num. AAA2702869

• Gene Names: DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
• Reactivity: Human
• Synonyms: Caspase Activated DNase (CAD); Human Caspase Activated DNase (CAD) ELISA Kit; DFF-B; DFFB; CPAN; DFF40; DFF2; DNA Fragmentation Factor 40kDa Beta Polypeptide; Caspase-activated deoxyribonuclease; Caspase-activated nuclease; CAD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Caspase Activated DNase (CAD).; No significant cross-reactivity or interference between Caspase Activated DNase (CAD) and analogues was observed.
• Sequence Length: 82

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.052ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase Activated DNase (CAD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase Activated DNase (CAD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase Activated DNase (CAD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase Activated DNase (CAD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase Activated DNase (CAD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase Activated DNase (CAD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37962942
• NCBI GeneID: 1677
• Molecular Weight: 11,482 Da
• NCBI Official Full Name: c-truncated caspase-activated DNase
• NCBI Official Synonym Full Names: DNA fragmentation factor subunit beta
• NCBI Official Symbol: DFFB
• NCBI Official Synonym Symbols: CAD; CPAN; DFF2; DFF40; DFF-40
• NCBI Protein Information: DNA fragmentation factor subunit beta
• UniProt Protein Name: DNA fragmentation factor subunit beta
• Protein Family: CAD protein
• UniProt Gene Name: DFFB
• UniProt Synonym Gene Names: CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

Uniprot Description

DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Deoxyribonuclease; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: cytosol; nuclear chromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: deoxyribonuclease activity; enzyme binding; identical protein binding; protein binding; protein domain specific binding

Biological Process: apoptotic chromosome condensation; apoptotic DNA fragmentation; DNA catabolic process; protein homooligomerization