Rabbit Epidermal growth factor ELISA Kit | EGF elisa kit

Rabbit Epidermal growth factor (EGF) ELISA Kit

Cat. Num. AAA282524

• Gene Names: EGF; URG; HOMG4
• Reactivity: Rabbit
• Synonyms: Epidermal growth factor; Rabbit Epidermal growth factor (EGF) ELISA Kit; EGF elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit EGF. No significant cross-reactivity or interference between Rabbit EGF and analogues was observed.
• Sequence Length: 53

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for EGF elisa kit

Epidermal growth factor (EGF) was discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice.EGF is a member of a family of growth factors that bind to the same 170 kDa receptor, including TGF-, vaccinia growth factor and amphiregulin. EGF is initially synthesized as a large (130 kDa) precursor molecule in which the mature, soluble EGF sequence (6 kDa) is located. The precursor, which functions as a source for soluble EGF, may also have a role in mediation of intercellular communication between cells displaying pro-EGF on their surfaces and cells with EGF receptors.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EGF in samples. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 374922206
• NCBI GeneID: 1950
• Molecular Weight: 129,746 Da
• NCBI Official Full Name: epidermal growth factor, partial
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: Epidermal growth factor
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; lysosomal membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; transmembrane receptor protein tyrosine kinase activator activity; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase activation (dimerization); peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; angiogenesis; DNA replication; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation; positive regulation of DNA binding; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitosis; branching morphogenesis of a tube; innate immune response; negative regulation of secretion; blood coagulation; positive regulation of phosphorylation

Disease: Hypomagnesemia 4, Renal