SDS-Page
(Figure. SDS-PAGE)
Interferon Alpha Active Protein | IFNa active protein
Active Interferon Alpha (IFNa)
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant Human, IFNa; Antibody: Rabbit Anti-IFNa Human Ab (PAA033Hu))
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant Human, IFNa; Antibody: Rabbit Anti-IFNa Human Ab (PAA033Hu))
Gene Sequencing
(Figure. Gene Sequencing (Extract))
Gene Sequencing
(Figure. Gene Sequencing (Extract))
Testing Data
(Interferon-alpha (IFN-), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN- has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFN- on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human IFN-. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37. Proliferation of A549 cells after incubation with IFN- for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant human IFN- for 48h. The result was shown in Figure 2. It was obvious that IFN- significantly inhibit cell viability of A549 cells. The ED50 is 3.4 ug/mL.(A) A549 cells cultured in DMEM, stimulated with 10ug/mL IFN- for 48h;(B) Unstimulated A549 cells cultured in DMEM for 48h.Figure 1. Inhibition of A549 cells proliferation after stimulated with IFN-)
Testing Data
(Interferon-alpha (IFN-), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN- has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFN- on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human IFN-. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37. Proliferation of A549 cells after incubation with IFN- for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant human IFN- for 48h. The result was shown in Figure 2. It was obvious that IFN- significantly inhibit cell viability of A549 cells. The ED50 is 3.4 ug/mL.(A) A549 cells cultured in DMEM, stimulated with 10ug/mL IFN- for 48h;(B) Unstimulated A549 cells cultured in DMEM for 48h.Figure 1. Inhibition of A549 cells proliferation after stimulated with IFN-)
Biological Activity Test
(Figure 2. Inhibition of A549 cells proliferation after stimulated with IFN-.)
Biological Activity Test
(Figure 2. Inhibition of A549 cells proliferation after stimulated with IFN-.)
NCBI and Uniprot Product Information
Accurate Molecular Mass: 48kDa
Uniprot Description
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
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Product Notes
The IFNa ifna1 (Catalog #AAA2122257) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Cys24~Leu181. AAA Biotech's Interferon Alpha can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the IFNa ifna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon Alpha, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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