Testing Data
GOPC-ROS1 recombinant protein
GOPC-ROS1 Mutant, Active
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Testing Data
Testing Data
Testing Data #2
Testing Data #2
Scientific Background: GOPC gene encodes a Golgi protein with a PDZ domain. Mice which are deficient in the GOPC protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms of GOPC have been found for this gene and GOPC gene locus is involved in translocations with other loci including c-ros oncogene 1 (ROS1) which result in the formation of the GOPC-ROS1 fusion protein that act as an oncogene. The ros1 gene encodes a proto-oncogenic protein which has been implicated in the pathogenesis of several types of cancer, including lung cancer and gliomas (1). GOPC-ROS1 fusion gene product has been identified in lung cancer (2).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.
Protein type: Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 6q22.1
Cellular Component: cytoplasm; Golgi apparatus; lysosomal membrane; membrane; plasma membrane; protein complex; trans-Golgi network transport vesicle
Molecular Function: protein binding
Biological Process: cytoplasmic sequestering of CFTR protein; negative regulation of anion channel activity; protein homooligomerization
Disease: Spermatogenic Failure 6
Research Articles on GOPC-ROS1
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Product Notes
The GOPC-ROS1 gopc (Catalog #AAA515650) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-419 (GOPC) - 1881-end (ROS1). AAA Biotech's GOPC-ROS1 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the GOPC-ROS1 gopc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOPC-ROS1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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