Mouse Endothelin-2 ELISA Kit | Edn2 elisa kit

Mouse Endothelin-2 ELISA Kit

Cat. Num. AAA765709

• Gene Names: EDN2; ET2; ET-2; PPET2
• Reactivity: Mouse
• Synonyms: Endothelin-2; Mouse Endothelin-2 ELISA Kit; Edn2/ET-2/Preproendothelin-2 (PPET2); Edn2 elisa kit

• Reactivity: Mouse
• Sequence Length: 178

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Edn2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Edn2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Edn2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Edn2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Edn2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503463
• NCBI GeneID: 1907
• NCBI Accession #: NP_001947.1
• NCBI GenBank Nucleotide #: NM_001956.4
• Molecular Weight: 19,960 Da
• NCBI Official Full Name: endothelin-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: endothelin 2
• NCBI Official Symbol: EDN2
• NCBI Official Synonym Symbols: ET2; ET-2; PPET2
• NCBI Protein Information: endothelin-2
• UniProt Protein Name: Endothelin-2
• Protein Family: Endothelin
• UniProt Gene Name: EDN2
• UniProt Synonym Gene Names: ET-2; PPET2
• UniProt Entry Name: EDN2_HUMAN

NCBI Description

This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

EDN2: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: cytosol; extracellular region; extracellular space; intracellular

Molecular Function: endothelin B receptor binding; hormone activity

Biological Process: alveolus development; artery smooth muscle contraction; calcium-mediated signaling; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; elevation of cytosolic calcium ion concentration; hormonal regulation of the force of heart contraction; inositol phosphate-mediated signaling; macrophage activation; macrophage chemotaxis; neutrophil chemotaxis; positive regulation of cell proliferation; positive regulation of heart rate; positive regulation of leukocyte chemotaxis; positive regulation of smooth muscle contraction; positive regulation of the force of heart contraction by chemical signal; prostaglandin biosynthetic process; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction; thermoregulation; vasoconstriction; vein smooth muscle contraction