Typical Testing Data/Standard Curve (for reference only)
Porcine Endothelin 2 (ET-2) ELISA Kit | ET-2 elisa kit
Porcine Endothelin 2 (ET-2) ELISA Kit
Gene Names
EDN2; ET2; ET-2; PPET2
Reactivity
Porcine
Synonyms
Endothelin 2 (ET-2); Porcine Endothelin 2 (ET-2) ELISA Kit; ET-2 elisa kit
Reactivity
Porcine
Sequence Length
178
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
2ng/ml - 600ng/ml
Sensitivity
1.02ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ET-2 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Endothelin 2 (also known as ET-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine ET-2 antibody. ET-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine ET-2 Antibody is added and binds to ET-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine ET-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine ET-2 antibody. ET-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine ET-2 Antibody is added and binds to ET-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine ET-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,960 Da
NCBI Official Full Name
endothelin-2 isoform 1 preproprotein
NCBI Official Synonym Full Names
endothelin 2
NCBI Official Symbol
EDN2
NCBI Official Synonym Symbols
ET2; ET-2; PPET2
NCBI Protein Information
endothelin-2
UniProt Protein Name
Endothelin-2
UniProt Gene Name
EDN2
UniProt Synonym Gene Names
ET-2; PPET2
NCBI Description
This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Uniprot Description
Endothelins are endothelium-derived vasoconstrictor peptides.
Research Articles on ET-2
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Product Notes
The Porcine ET-2 edn2 (Catalog #AAA1600902) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600902 ELISA Kit recognizes Porcine ET-2. It is sometimes possible for the material contained within the vial of "Endothelin 2 (ET-2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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