Human cytochrome c, somatic ELISA Kit | CYCS elisa kit

Human Cytochrome-C, Cyt-C ELISA Kit

Cat. Num. AAA704746

• Gene Names: CYCS; CYC; HCS; THC4
• Reactivity: Human
• Synonyms: cytochrome c; somatic; Human Cytochrome-C; Cyt-C ELISA Kit; CYC; HCS; THC4; OTTHUMP00000202043; OTTHUMP00000202044; CYCS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CYCS. No significant cross-reactivity or interference between Human CYCS and analogues was observed.
• Sequence Length: 105

• Samples: Serum, plasma, Cell lysates
• Detection Range: 7.8 ng/ml-500 ng/ml
• Sensitivity: 1.95 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYCS elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyt-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cyt-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Cyt-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cyt-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11128019
• NCBI GeneID: 54205
• NCBI Accession #: NP_061820.1
• NCBI GenBank Nucleotide #: NM_018947.5
• UniProt Accession #: P99999; P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1
• Molecular Weight: 11,749 Da
• NCBI Official Full Name: cytochrome c
• NCBI Official Synonym Full Names: cytochrome c, somatic
• NCBI Official Symbol: CYCS
• NCBI Official Synonym Symbols: CYC; HCS; THC4
• NCBI Protein Information: cytochrome c
• UniProt Protein Name: Cytochrome c
• Protein Family: Cytochrome
• UniProt Gene Name: CYCS
• UniProt Synonym Gene Names: CYC
• UniProt Entry Name: CYC_HUMAN

NCBI Description

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Uniprot Description

Function: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.