Typical Testing Data/Standard Curve (for reference only)
()
Human Endothelin-1 ELISA Kit | EDN1 elisa kit
Human Endothelin-1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin-1, During the reaction, Endothelin-1 in the sample or standard competes with a fixed amount of biotin- labeled Endothelin-1 for sites on a pre-coated Monoclonal antibody specific to Endothelin-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Endothelin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Uniprot Description
EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6p24.1
Cellular Component: cytoplasm; extracellular region; extracellular space; transport vesicle
Molecular Function: cytokine activity; endothelin A receptor binding; endothelin B receptor binding; hormone activity; protein binding
Biological Process: artery smooth muscle contraction; calcium-mediated signaling; cell surface receptor linked signal transduction; cell-cell signaling; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; inositol phosphate-mediated signaling; leukocyte activation; negative regulation of blood coagulation; negative regulation of cellular protein metabolic process; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; neutrophil chemotaxis; nitric oxide transport; peptide hormone secretion; phosphoinositide 3-kinase cascade; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of heart rate; positive regulation of hormone secretion; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of nitric oxide biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; prostaglandin biosynthetic process; protein kinase C deactivation; regulation of systemic arterial blood pressure by endothelin; vasoconstriction; vein smooth muscle contraction
Disease: Auriculocondylar Syndrome 3; Question Mark Ears, Isolated
Research Articles on EDN1
Similar Products
Product Notes
The Human EDN1 edn1 (Catalog #AAA9425688) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9425688 ELISA Kit recognizes Human EDN1. It is sometimes possible for the material contained within the vial of "Endothelin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
