EBAG9 recombinant protein

EBAG9, 28-213aa, Human,His tag, E Coli

Cat. Num. AAA203246

• Gene Names: EBAG9; EB9; PDAF
• Applications: SDS-Page
• Purity: > 90% by SDS PAGE
• Synonyms: EBAG9; 28-213aa; Human; His tag; E Coli; Estrogen receptor binding site associated antigen 9; EB9; PDAF; RCAS1; Estrogen receptor binding site associated; antigen 9 BAG9; Cancer associated surface antigen; Cancer associated surface antigen RCAS1; EBAG 9; Estrogen receptor binding fragment associated gene 9 protein; RCAS 1; Receptor binding cancer antigen expressed on SiSo cells.; EBAG9 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS PAGE
• Form/Format: Liquid in 20 mM Tris pH 8.0, 1 mM DTT, 2 mM EDTA, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MRSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS
• Sequence Length: 213

• Applicable Applications for EBAG9 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EBAG9 recombinant protein

Estrogen receptor binding site associated antigen 9, also known as EBAG9, is a type III transmembrane protein that is predominantly located in the Golgi. This protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers, such as advanced breast and prostate cancers. As the EBAG9 acts to inhibit the growth of receptor-binding cells and induced apoptosis of immune cells, cancer cells might evade immune surveillance. So immunodetection of EBAG9 expression can be a negative prognostic indicator. Recombinant human EBAG9 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for EBAG9 recombinant protein

Cancer

References

Kumagai J., et al., (2009) Int J Cancer.124 (4):816-26.; Yoshida S., et al., (2008) J Rheumatol. 35 (9):1716-22.;

NCBI and Uniprot Product Information

• NCBI GI #: 4758230
• NCBI GeneID: 9166
• NCBI Accession #: NP_004206
• NCBI GenBank Nucleotide #: NM_004215.4
• UniProt Accession #: O00559; Q5Y8C7; Q6IB20; Q9BS76; A8K3N6
• Molecular Weight: 23.4 kDa (207 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: receptor-binding cancer antigen expressed on SiSo cells
• NCBI Official Synonym Full Names: estrogen receptor binding site associated, antigen, 9
• NCBI Official Symbol: EBAG9
• NCBI Official Synonym Symbols: EB9; PDAF
• NCBI Protein Information: receptor-binding cancer antigen expressed on SiSo cells
• UniProt Protein Name: Receptor-binding cancer antigen expressed on SiSo cells
• Protein Family: Receptor-binding cancer antigen
• UniProt Gene Name: EBAG9
• UniProt Synonym Gene Names: RCAS1

NCBI Description

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]

Uniprot Description

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.