Rabbit anti-Human FOLR1 Polyclonal Antibody | anti-FOLR1 antibody

FOLR1, NT (FOLR1, FOLR, Folate receptor alpha, Adult folate-binding protein, Folate receptor 1, Folate receptor, adult, KB cells FBP, Ovarian tumor-associated antigen MOv18) (FITC)

Cat. Num. AAA6299602

• Gene Names: FOLR1; FBP; FOLR
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: FOLR1; Polyclonal Antibody; NT (FOLR1; FOLR; Folate receptor alpha; Adult folate-binding protein; Folate receptor 1; Folate receptor; adult; KB cells FBP; Ovarian tumor-associated antigen MOv18) (FITC); Ovarian tumor-associated antigen MOv18; anti-FOLR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FOLR1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FOLR1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 33-68 in the N-terminal region of human FOLR1, Swiss/UniProt Accession: P15328
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Hela cell line lysates (35ug/lane) usingMBS6009693. This demonstrates MBS6009693 detected the FOLR1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human kidney tissue using MBS6009693 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells (right histogram) using MBS6009693 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FOLR1 antibody

Antibodies; Binding Proteins

References

1. Sivakumaran, S., et al. J. Steroid Biochem. Mol. Biol. 122(5):333-340(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. O'Byrne, M.R., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):689-694(2010). 4. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 5. Elwood, P.C., et al. Biochemistry 36(6):1467-1478(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4758400
• NCBI GeneID: 2348
• NCBI Accession #: NP_000793.1
• NCBI GenBank Nucleotide #: NM_000802.3
• UniProt Accession #: P15328; Q53EW2; Q6FGT8; Q6LC90; Q9UCT2
• Molecular Weight: 29,819 Da
• NCBI Official Full Name: folate receptor alpha
• NCBI Official Synonym Full Names: folate receptor 1 (adult)
• NCBI Official Symbol: FOLR1
• NCBI Official Synonym Symbols: FBP; FOLR
• NCBI Protein Information: folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18
• UniProt Protein Name: Folate receptor alpha
• Protein Family: Folate receptor
• UniProt Gene Name: FOLR1
• UniProt Synonym Gene Names: FOLR; FR-alpha; FBP
• UniProt Entry Name: FOLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

FOLR1: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain- specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. Belongs to the folate receptor family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q13.3-q14.1

Cellular Component: anchored to external side of plasma membrane; cell surface; membrane; clathrin-coated vesicle; brush border membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; endosome

Molecular Function: folic acid transporter activity; receptor activity; folic acid binding

Biological Process: receptor-mediated endocytosis; folic acid metabolic process; folic acid transport

Disease: Neurodegeneration Due To Cerebral Folate Transport Deficiency