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Western Blot (WB) (EBAG9 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EBAG9 antibody detected the EBAG9 protein (arrow).)

Rabbit EBAG9 Polyclonal Antibody | anti-EBAG9 antibody

EBAG9 Antibody (Center)

Gene Names
EBAG9; EB9; PDAF
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EBAG9; Polyclonal Antibody; EBAG9 Antibody (Center); Receptor-binding cancer antigen expressed on SiSo cells; Cancer-associated surface antigen RCAS1; Estrogen receptor-binding fragment-associated gene 9 protein; RCAS1; anti-EBAG9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EBAG9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human EBAG9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
88-117
Sequence Length
258
Applicable Applications for anti-EBAG9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(EBAG9 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EBAG9 antibody detected the EBAG9 protein (arrow).)

Western Blot (WB) (EBAG9 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EBAG9 antibody detected the EBAG9 protein (arrow).)
Related Product Information for anti-EBAG9 antibody
This gene was identified as an estrogen-responsive gene.
Regulation of transcription by estrogen is mediated by estrogen
receptor which binds to the estrogen-responsive element (ERE) found
in the 5'-flanking region of this gene. The encoded protein is a
tumor-associated antigen that is expressed at high frequency in a
variety of cancers. Two transcript variants differing in the 5'
UTR, but encoding the same protein, have been identified for this
gene.
Product Categories/Family for anti-EBAG9 antibody
References
Wolf, J., et al. FASEB J. 24(10):4000-4019(2010)
Sonoda, K., et al. Exp. Cell Res. 316(11):1795-1803(2010)
Ali-Fehmi, R., et al. Cancer Biomark 6(1):33-48(2010)
Yang, Y.C., et al. Zhonghua Wai Ke Za Zhi 47(13):999-1001(2009)
Adamek, D., et al. Folia Neuropathol 47(3):240-246(2009)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24377
NCBI Official Full Name
receptor-binding cancer antigen expressed on SiSo cells
NCBI Official Synonym Full Names
estrogen receptor binding site associated, antigen, 9
NCBI Official Symbol
EBAG9
NCBI Official Synonym Symbols
EB9; PDAF
NCBI Protein Information
receptor-binding cancer antigen expressed on SiSo cells
UniProt Protein Name
Receptor-binding cancer antigen expressed on SiSo cells
UniProt Gene Name
EBAG9
UniProt Synonym Gene Names
RCAS1
UniProt Entry Name
RCAS1_HUMAN

NCBI Description

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]

Uniprot Description

RCAS1: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE)-like proteases. Homodimer. By estrogen. Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: Golgi membrane; Golgi apparatus; focal adhesion; integral to membrane; plasma membrane; secretory granule

Molecular Function: apoptotic protease activator activity

Biological Process: apoptosis; regulation of cell growth

Research Articles on EBAG9

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Product Notes

The EBAG9 ebag9 (Catalog #AAA9202634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 88-117. The EBAG9 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's EBAG9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the EBAG9 ebag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBAG9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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