EBAG9 recombinant protein

EBAG9 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121611

• Gene Names: EBAG9; EB9; PDAF
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EBAG9; EBAG9 Protein; Human; Recombinant (His Tag); Human RCAS1/EBAG9 Protein (His Tag); EB9 Protein; PDAF Protein; estrogen receptor binding site associated; antigen; 9; EBAG9 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Arg28-Ser213

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human EBAG9 (O00559)(Arg28-Ser213) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EBAG9 recombinant protein

Background: RCAS1, also known as EBAG9, is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. RCAS1 gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of RCAS1 gene. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for RCAS1 gene. EBAG9 may participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

References

Ohshima K. et al., 2001, Clin Exp Immunol. 123 (3): 481-6. Ikeda K. et al., 2000, Biochem Biophys Res Commun. 273 (2): 654-60. Nakashima M. et al., 1999, Nat Med. 5 (8): 938-42.

NCBI and Uniprot Product Information

• NCBI GI #: 8928283
• NCBI GeneID: 9166
• UniProt Accession #: O00559; Q5Y8C7; Q6IB20; Q9BS76; A8K3N6
• Molecular Weight: 213
• NCBI Official Full Name: Receptor-binding cancer antigen expressed on SiSo cells
• NCBI Official Synonym Full Names: estrogen receptor binding site associated, antigen, 9
• NCBI Official Symbol: EBAG9
• NCBI Official Synonym Symbols: EB9; PDAF
• NCBI Protein Information: receptor-binding cancer antigen expressed on SiSo cells; cancer-associated surface antigen RCAS1; estrogen receptor-binding fragment-associated gene 9 protein
• UniProt Protein Name: Receptor-binding cancer antigen expressed on SiSo cells
• Protein Family: Receptor-binding cancer antigen
• UniProt Gene Name: EBAG9
• UniProt Synonym Gene Names: RCAS1
• UniProt Entry Name: RCAS1_HUMAN

NCBI Description

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]

Uniprot Description

RCAS1: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE)-like proteases. Homodimer. By estrogen. Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: Golgi membrane; Golgi apparatus; focal adhesion; plasma membrane; integral to membrane; secretory granule

Molecular Function: apoptotic protease activator activity

Biological Process: apoptosis; regulation of cell growth