HMG-CoA Reductase Catalytic Domain Recombinant Protein | HMGCR recombinant protein

HMG-CoA Reductase Catalytic Domain (GST Tagged), human recombinant

Cat. Num. AAA845121

• Gene Names: HMGCR; LDLCQ3
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: HMG-CoA Reductase Catalytic Domain; HMG-CoA Reductase Catalytic Domain (GST Tagged); human recombinant; 3-Hydroxy-3-methylglutaryl-CoA reductase; HMGR; HMGCR; HMGCR recombinant protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: Lyophilized from proprietary buffer containing BSA; Appearance: Lyophilized
• Sequence Length: 888

• Applicable Applications for HMGCR recombinant protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Briefly spin down the vial and reconstitute in 50% glycerol in PBS to 0.5-1.0 mg/ml and store at -80 degree C.
• Activity (Specifications/test method): The specific activity was determined to be > 1.0 Unit/mg by MyBioSource's HMG-CoA reductase Activity/Inhibitor Screening Kit.
• Results: > 1.0 Unit/mg
• Unit Definition: One unit is defined as the amount of enzyme required to convert 1.0 mumole of NADPH to NADP+ per minute at 37 degree C
• Handling: Centrifuge the vial prior to opening.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Testing Data

Related Product Information for HMGCR recombinant protein

Background: HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is a transmembrane protein involved in the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. Since HMG-CoA reductase catalyzes the rate limiting step in cholesterol synthesis, it is a primary target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.

Product Categories/Family for HMGCR recombinant protein

Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4557643
• NCBI GeneID: 3156
• NCBI Accession #: NP_000850.1
• NCBI GenBank Nucleotide #: NM_000859.2
• UniProt Accession #: P04035; Q8N190; B7Z3Y9
• Molecular Weight: 75.9 kDa (426-888 aa, NT GST Tag)
• NCBI Official Full Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform 1
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-CoA reductase
• NCBI Official Symbol: HMGCR
• NCBI Official Synonym Symbols: LDLCQ3
• NCBI Protein Information: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• UniProt Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• UniProt Gene Name: HMGCR
• UniProt Synonym Gene Names: HMG-CoA reductase
• UniProt Entry Name: HMDH_HUMAN

NCBI Description

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q13.3-q14

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; coenzyme binding

Biological Process: coenzyme A metabolic process; myoblast differentiation; negative regulation of MAP kinase activity; ubiquinone metabolic process; response to ethanol; isoprenoid biosynthetic process; negative regulation of vasodilation; positive regulation of smooth muscle cell proliferation; visual learning; positive regulation of skeletal muscle development; cellular lipid metabolic process; cholesterol biosynthetic process; protein tetramerization; response to nutrient; positive regulation of stress-activated MAPK cascade; aging