Nicotinamide Nucleotide Adenylyltransferase 1 Active Protein | NMNAT1 active protein

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1, Active

Cat. Num. AAA146773

• Gene Names: NMNAT1; LCA9; NMNAT; PNAT1
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Nicotinamide Nucleotide Adenylyltransferase 1; Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1; Active; NMNAT1 Human; NMNAT; NMNAT1; PNAT1; Nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; Nicotinate-nucleotide adenylyltransferase 1; NaMN adenylyltransferase 1; EC=2.7.7.1; EC=2.7.7.18; Nicotinamide Nucleotide Adenylyltransferase 1 Human Recombinant; NMNAT1 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The NMNAT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT and 1mM EDTA.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQSIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAKT
• Sequence Length: 279

• Biological Activity: Specific activity is > 7, 000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Related Product Information for NMNAT1 active protein

Description: NMNAT1 Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-279 a.a.) and having a molecular mass of 36 kDa. The NMNAT1 is fused to a 36 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Introduction: NMNAT1 enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. NMNAT1 is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.

Product Categories/Family for NMNAT1 active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 663071195
• NCBI GeneID: 64802
• NCBI Accession #: NP_001284707.1
• NCBI GenBank Nucleotide #: NM_001297778.1
• UniProt Accession #: Q9HAN9; Q8TAE9; Q9H247; Q9H6B6; B1AN63
• Molecular Weight: 31,932 Da
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 1
• NCBI Official Symbol: NMNAT1
• NCBI Official Synonym Symbols: LCA9; NMNAT; PNAT1
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Gene Name: NMNAT1
• UniProt Synonym Gene Names: NMNAT; NMN/NaMN adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1

NCBI Description

This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]

Uniprot Description

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+ (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD+ and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+ (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults ().