FGF-2 (basic) Active Protein | FGF-2 active protein

Mouse FGF-2 (basic)

Cat. Num. AAA691736

• Gene Names: Fgf2; Fgfb; bFGF; Fgf-2
• Reactivity: Mouse
• Purity: > 98% by SDS-PAGE & silver stain
• Synonyms: FGF-2 (basic); Mouse FGF-2 (basic); Recombinant Mouse Fibroblast Growth Factor-2 (basic); basic Fibroblast growth factor (bFGF); Heparin binding growth factor-2 (HBGF-2); FGF-2 active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: > 98% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: ALPEDGG
• Sequence Length: 144

• Endotoxin Level: < 0.1ng per ug of mouse FGF-2
• Buffer: PBS
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Basic FGF can be stored in high-salt buffer (PBS, 1M NaCl) at 4 degree C for 2-4 weeks.

Testing Data

Testing Data #2

Related Product Information for FGF-2 active protein

The FGF family is composed of at least 23 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors (HB-GF). Until the structure of basic fibroblast growth factor (bFGF/FGF-2) was determined, a number of synonyms was used to describe this growth factor. As is often the case, the nomenclature reflected the observed activities reported by individual groups. Basic FGF has been reported as leukemia growth factor, macrophage growth factor, endothelial growth factor and tumor angiogenesis factor. The eventual isolation and characterization of bFGF was done from soluble brain extracts. bFGF was found to have a molecular mass of 16.5 kDa and to be 154 amino acids in length. Interestingly, bFGF contains no hydrophobic leader sequence previously thought to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. FGF's induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. Perhaps one of the most potentially significant applications of bFGF is related to its reported ability to induce angiogenesis.

Product Categories/Family for FGF-2 active protein

Cytokines & Growth Factors

References

1. Coulier F et al, J Mol Evol 44:43, 1997 2. Fernig D et al, Prog Growth Factor Res 5:353, 1994 3. Presta M et al, Cytokine Growth Factor Rev 16:159, 2005 4. Claus P et al, J Biol Chem 278:479, 2003 5. Quarto N et al, Gene 356:49, 2005 6. Tsuneto M et al, Biochem Biophys Res Comm 335:1239, 2005 7. Hebert JM et al, Dev Biol 138:454, 1990

NCBI and Uniprot Product Information

• NCBI GI #: 7106315
• NCBI GeneID: 14173
• NCBI Accession #: NP_032032.1
• NCBI GenBank Nucleotide #: NM_008006.2
• UniProt Accession #: P15655
• Molecular Weight: 16.35 kDa
• NCBI Official Full Name: fibroblast growth factor 2
• NCBI Official Synonym Full Names: fibroblast growth factor 2
• NCBI Official Symbol: Fgf2
• NCBI Official Synonym Symbols: Fgfb; bFGF; Fgf-2
• NCBI Protein Information: fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2
• UniProt Protein Name: Fibroblast growth factor 2
• UniProt Gene Name: Fgf2
• UniProt Synonym Gene Names: Fgf-2; FGF-2; bFGF; HBGF-2
• UniProt Entry Name: FGF2_MOUSE

Uniprot Description

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro

By similarity.

Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2

By similarity. Ref.3

Subcellular location: Secreted

By similarity. Nucleus

By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane

By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57

By similarity.

Post-translational modification: Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion

By similarity.

Sequence similarities: Belongs to the heparin-binding growth factors family.