FGF-2 (basic) Active Protein | FGF-2 active protein

Mouse FGF-2 (basic)

Cat. Num. AAA691614

• Gene Names: Fgf2; Fgfb; bFGF; Fgf-2
• Reactivity: Mouse
• Purity: > 98% by SDS-PAGE & silver stain
• Synonyms: FGF-2 (basic); Mouse FGF-2 (basic); basic Fibroblast growth factor (bFGF); Heparin binding growth factor-2 (HBGF-2); FGF-2 active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: > 98% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: ALPEDGG
• Sequence Length: 144

• Endotoxin Level: < 0.1ng per ug of mouse FGF-2
• Buffer: PBS
• Reconstitution: The lyophilized basic FGF should be reconstituted in water containing at least 0.1% human or bovine serum albumin to a concentration not lower than 10 ug/ml.
• Length: 144
• Result by N-terminal sequencing: ALPEDGG
• Sequence: ALPEDGGAAFPPGHFKDPKR LYCKNGGFFLRIHPDGRVDG VREKSDPHVKLQ; LQAEERGVVSIKGVCANRYL AMKEDGRLLASKCVTEECFF FERLESNNYNTY; RSRKYSSWYVALKRTGQYKL GSKTGPGQKAILFLPMSAKS
• Biological Activity: The ED₅₀ for stimulation of cell proliferation by human umbilical vein endothelial cells for mouse FGF-2 has been determined to be in the range of 0.1-2ng/ml.
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Basic FGF can be stored in high-salt buffer (PBS, 1M NaCl) at 4 degree C for 2-4 weeks.

Testing Data

Testing Data #2

Related Product Information for FGF-2 active protein

FGF basic (FGF2, HBGF2) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH3T3 cells (5). The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic (6,7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

Product Categories/Family for FGF-2 active protein

Cytokines & Growth Factors

References

1. Coulier F et al, J Mol Evol 44:43, 1997 2. Fernig D et al, Prog Growth Factor Res 5:353, 1994 3. Presta M et al, Cytokine Growth Factor Rev 16:159, 2005 4. Claus P et al, J Biol Chem 278:479, 2003 5. Quarto N et al, Gene 356:49, 2005 6. Tsuneto M et al, Biochem Biophys Res Comm 335:1239, 2005 7. Hebert JM et al, Dev Biol 138:454, 1990

NCBI and Uniprot Product Information

• NCBI GI #: 7106315
• NCBI GeneID: 14173
• NCBI Accession #: NP_032032.1
• NCBI GenBank Nucleotide #: NM_008006.2
• UniProt Accession #: P15655
• Molecular Weight: 16.35 kDa
• NCBI Official Full Name: fibroblast growth factor 2
• NCBI Official Synonym Full Names: fibroblast growth factor 2
• NCBI Official Symbol: Fgf2
• NCBI Official Synonym Symbols: Fgfb; bFGF; Fgf-2
• NCBI Protein Information: fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2
• UniProt Protein Name: Fibroblast growth factor 2
• UniProt Gene Name: Fgf2
• UniProt Synonym Gene Names: Fgf-2; FGF-2; bFGF; HBGF-2
• UniProt Entry Name: FGF2_MOUSE

Uniprot Description

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro

By similarity.

Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2

By similarity. Ref.3

Subcellular location: Secreted

By similarity. Nucleus

By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane

By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57

By similarity.

Post-translational modification: Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion

By similarity.

Sequence similarities: Belongs to the heparin-binding growth factors family.