Western Blot
(Western blot analysis using HLADR beta antibodyWestern blot analysis of 30 ug whole cell lysate (A:Raji) using a 12 % SDS PAGE gel and HLADR beta antibody at a dilution of 1:1000)
Rabbit HLADR beta Polyclonal Antibody | anti-HLADR beta antibody
HLADR beta antibody
Applications
Western Blot
Purity
HLADR beta antibody was purified by antigen-affinity chromatography
Synonyms
HLADR beta; Polyclonal Antibody; HLADR beta antibody; Polyclonal HLADR beta; Anti-HLADR beta; MHC class II antigen DRB4; DR-13; DR14; leukocyte antigen; HLA-DRB4; DR13; MHC class2 antigen; MHC HLA DR-beta chain; human leucocyte antigen DRB4; DR-14; HLA-DR4B; major histocompatibility complex class II DR beta 4; HLA class II histocompatibility antigen DR beta 4 chain; DRB4; anti-HLADR beta antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
HLADR beta antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-HLADR beta antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:3000
Biological Significance
HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
Cross-Reactivity
Human
Immunogen
HLADR beta antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 266 of HLA DR beta
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot
(Western blot analysis using HLADR beta antibodyWestern blot analysis of 30 ug whole cell lysate (A:Raji) using a 12 % SDS PAGE gel and HLADR beta antibody at a dilution of 1:1000)
Western Blot
(Western blot analysis using HLADR beta antibodyWestern blot analysis of 30 ug whole cell lysate (A:Raji) using a 12 % SDS PAGE gel and HLADR beta antibody at a dilution of 1:1000)
Related Product Information for anti-HLADR beta antibody
Affinity purified Rabbit polyclonal HLADR beta antibody
Product Categories/Family for anti-HLADR beta antibody
Similar Products
Product Notes
The HLADR beta (Catalog #AAA837261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HLADR beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:3000. Researchers should empirically determine the suitability of the HLADR beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLADR beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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