IL-15 active protein

Human IL-15 Protein

Cat. Num. AAA9718675

• Gene Names: IL15; IL-15
• Purity: >95% as determined by SDS-PAGE.
• Synonyms: IL-15; Human IL-15 Protein; Recombinant Human IL15 Protein (Interleukin-15) expressed in E. coli; Interleukin 15; Interleukin-15; IL15; IL-15 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile 50 mM Tris, 200 mM NaCl, pH 8.0.
• Sequence: Amino acid sequence derived from human IL15 (NP_000576.1) (Asn49-Ser162) was expressed.
• Sequence Length: 162

• Species: Human
• Endotoxin: The Endotoxin level is less than 0.1 EU per 1mug cytokine as determined by the LAL method.
• Protein Length: The recombinant human IL15 consists of 114 amino acids and predicts a molecular mass of 12.8 kDa.
• Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The EC50 for this effect is typically 0.8-4 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL15 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-15 protein.)

Related Product Information for IL-15 active protein

The protein encoded by IL-15 is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 10835153
• NCBI GeneID: 3600
• NCBI Accession #: NP_000576.1
• NCBI GenBank Nucleotide #: NP_000576.1
• UniProt Accession #: P40933
• Molecular Weight: 12.8 kDa
• NCBI Official Full Name: interleukin-15 isoform 1 preproprotein
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: IL15
• NCBI Official Synonym Symbols: IL-15
• NCBI Protein Information: interleukin-15

NCBI Description

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]