Follistatin Active Protein | mFST active protein

Recombinant Mouse Follistatin

Cat. Num. AAA144799

• Gene Names: Fst; FS; AL033346
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Follistatin; Recombinant Mouse Follistatin; FST Mouse; Follistatin Mouse Recombinant; FST; FS; Activin-binding protein; AL033346; mFST active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The Mouse Follistatin is lyophilized from 10mM Na2PO4 and 50mM NaCl, pH 7.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MGNCWLRQAK NGRCQVLTKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DS
• Sequence Length: 344

• Solubility: It is recommended to reconstitute the lyophilized Follistatin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50, determined by the dose-dependent neutralization of 7.5ng/ml human Activin-A on MCP-11 cells, is 0.13-0.19 ug/ml.
• Preparation and Storage: Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FST should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mFST active protein

Description: Follistatin Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 289 amino acids and having a total molecular mass of 31.6kDa.The FST is purified by proprietary chromatographic techniques.

Introduction: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (fsh).

Product Categories/Family for mFST active protein

Cytokines and Growth Factors; Cytokines; Follistatin

NCBI and Uniprot Product Information

• NCBI GI #: 672890584
• NCBI GeneID: 14313
• NCBI Accession #: NP_001288302.1
• NCBI GenBank Nucleotide #: NM_001301373.1
• UniProt Accession #: P47931; A6H6P0
• Molecular Weight: 37,866 Da
• NCBI Official Full Name: follistatin FST315
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: Fst
• NCBI Official Synonym Symbols: FS; AL033346
• NCBI Protein Information: follistatin; activin-binding protein
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: Fst
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_MOUSE

NCBI Description

The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH)

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; activin binding

Biological Process: BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; hair follicle morphogenesis; gamete generation; hemopoietic progenitor cell differentiation; keratinocyte proliferation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; skeletal development; female gonad development; odontogenesis of dentine-containing teeth