Western Blot (WB)
(Western Blot: Sample: Bovine Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine TF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Cattle Tissue Factor Polyclonal Antibody | anti-TF antibody
Polyclonal Antibody to Tissue Factor (TF)
Gene Names
F3; TF
Reactivity
Cattle
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Tissue Factor; Polyclonal Antibody; Polyclonal Antibody to Tissue Factor (TF); anti-TF antibody
Host
Rabbit
Reactivity
Cattle
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TF. It has beenselected for its ability to recognize TF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TDVVV AYNITWKSTN FKTILEWEPK PINHVYTVQI SPRLGNWKNK CFYTTNTECD VTDEIVKNVR ETYLARVLSY PADTSSSTVE PPFTNSPEFT PYLETNLGQP TIQSFEQVGT KLNVTVQDAR TLVRANSAFL SLRDVFGKDL NYTLYYWKAS STGKKKATTN TNGFLIDVDK GENYCFHVQA VILSRRVNQK SPESPIKCTS HEKVLSTELF FIIGTVMLVI IIFIVVLSVS LHKCRKVRAE RSGKENTPLN AA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TDVVV AYNITWKSTN FKTILEWEPK PINHVYTVQI SPRLGNWKNK CFYTTNTECD VTDEIVKNVR ETYLARVLSY PADTSSSTVE PPFTNSPEFT PYLETNLGQP TIQSFEQVGT KLNVTVQDAR TLVRANSAFL SLRDVFGKDL NYTLYYWKAS STGKKKATTN TNGFLIDVDK GENYCFHVQA VILSRRVNQK SPESPIKCTS HEKVLSTELF FIIGTVMLVI IIFIVVLSVS LHKCRKVRAE RSGKENTPLN AA
Sequence Length
292
Applicable Applications for anti-TF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
TF (Thr36~Ala292)
Organism Species
Bos taurus; Bovine (Cattle)
Cross Reactivity
Bovine
Conjugate
No Conjugate
Immunogen
Recombinant TF (Thr36~Ala292) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043365
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Bovine Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine TF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Bovine Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine TF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,475 Da
NCBI Official Full Name
tissue factor
NCBI Official Synonym Full Names
coagulation factor III, tissue factor
NCBI Official Symbol
F3
NCBI Official Synonym Symbols
TF
NCBI Protein Information
tissue factor
UniProt Protein Name
Tissue factor
Protein Family
UniProt Gene Name
F3
UniProt Synonym Gene Names
TF
Uniprot Description
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
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Product Notes
The TF f3 (Catalog #AAA2026725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tissue Factor (TF) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Tissue Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the TF f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TDVVV AYNITWKSTN FKTILEWEPK PINHVYTVQI SPRLGNWKNK CFYTTNTECD VTDEIVKNVR ETYLARVLSY PADTSSSTVE PPFTNSPEFT PYLETNLGQP TIQSFEQVGT KLNVTVQDAR TLVRANSAFL SLRDVFGKDL NYTLYYWKAS STGKKKATTN TNGFLIDVDK GENYCFHVQA VILSRRVNQK SPESPIKCTS HEKVLSTELF FIIGTVMLVI IIFIVVLSVS LHKCRKVRAE RSGKENTPLN AA. It is sometimes possible for the material contained within the vial of "Tissue Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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