Mucosae-Associated Epithelial Chemokine Active Protein | MEC active protein

Recombinant Human Mucosae-Associated Epithelial Chemokine (CCL28)

Cat. Num. AAA143840

• Gene Names: CCL28; MEC; CCK1; SCYA28
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Mucosae-Associated Epithelial Chemokine; Recombinant Human Mucosae-Associated Epithelial Chemokine (CCL28); CCL28 Human; Mucosae-Associated Epithelial Chemokine Human Recombinant (CCL28); MEC; CCK1; SCYA28; MGC71902; CCL28; C-C motif chemokine 28; Small-inducible cytokine A28; Mucosae-associated epithelial chemokine; Protein CCK1; MEC active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM PBS and 150mM NaCl.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY
• Sequence Length: 127

• Solubility: It is recommended to reconstitute the lyophilized CCL28 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Determined by its ability to chemoattract human lymphocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.
• Preparation and Storage: Lyophilized CCL28 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL28 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for MEC active protein

Description: CCL28 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 108 amino acids and having a molecular mass of 12.3 kDa. The CCL28 is purified by proprietary chromatographic techniques.

Introduction: CCL28 is part of the subfamily of small cytokine CC genes. CCL28 shows chemotactic activity for resting CD4 or CD8 T cells and eosinophils. CCL28 binds to chemokine receptors CCR3 and CCR10. CCL28 is involved in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. CCL28 mediates mucosal immunity in HIV exposure and infection. CCL28 is involved in the pathogenesis of inflammatory skin diseases. Human CCL28 cDNA encodes a 127 amino acid residue precursor protein with a putative 22 amino acid residue signal peptide that is cleaved to produce the 105 amino acid residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% amino acid identity in their mature regions. CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues.

Product Categories/Family for MEC active protein

Chemokines; MEC (CCL28)

NCBI and Uniprot Product Information

• NCBI GI #: 687569447
• NCBI GeneID: 56477
• NCBI Accession #: NP_001288802.1
• NCBI GenBank Nucleotide #: NM_001301873.1
• UniProt Accession #: Q9NRJ3; D7RIE7
• Molecular Weight: 12,375 Da
• NCBI Official Full Name: C-C motif chemokine 28 isoform a
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 28
• NCBI Official Symbol: CCL28
• NCBI Official Synonym Symbols: MEC; CCK1; SCYA28
• NCBI Protein Information: C-C motif chemokine 28; CC chemokine CCL28; chemokine (C-C motif) ligand 28 splice variant chi; mucosae-associated epithelial chemokine; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys), member 28; small-inducible cytokine A28
• UniProt Protein Name: C-C motif chemokine 28
• Protein Family: CysO-cysteine peptidase
• UniProt Gene Name: CCL28
• UniProt Synonym Gene Names: SCYA28; MEC
• UniProt Entry Name: CCL28_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Chemokine

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: elevation of cytosolic calcium ion concentration; immune response; chemotaxis; positive regulation of cell-matrix adhesion; response to nutrient