T4 DNA Ligase
T4 DNA Ligase (High Conc.)
Applications
Cloning Ligation, Adaptor Ligation
Synonyms
T4 DNA Ligase; T4 DNA Ligase (High Conc.); T4 DNA Ligase ligase
Host
E Coli
Form/Format
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH7.4 at 25 degree C
Concentration
2,000,000 U/ml (varies by lot)
Applicable Applications for T4 DNA Ligase ligase
Cloning Ligation, Adaptor Ligation
Source Note
An E Coli strain that carries the T4 DNA ligase gene.
Unit Definition
One unit is defined as the amount of enzyme required to give 50% ligase of HindIII fragments of lambda DNA (5' DNA termini concentration of 0.12 muM, 300- mug/ml) in a total reaction volume of 20 mul in 30 minutes at 16 degree C in 1X T4 DNA Ligase Reaction Buffer.
Reaction Conditions
1X T4 DNA Ligase Reaction Buffer.
Reaction Buffer or Mix
1X T4 DNA Ligase Reaction Buffer:
50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH7.5 at 25 degree C
50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH7.5 at 25 degree C
Heat Inactivation
65 degree C for 10 min.
Preparation and Storage
Store at -20 degree C.
Related Product Information for T4 DNA Ligase ligase
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. It is applicable to cloning of restriction fragments and joining linkers and adapters to blunt-ended DNA
.
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Product Notes
The T4 DNA Ligase (Catalog #AAA9141678) is a Ligase produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T4 DNA Ligase can be used in a range of immunoassay formats including, but not limited to, Cloning Ligation, Adaptor Ligation. Researchers should empirically determine the suitability of the T4 DNA Ligase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T4 DNA Ligase, Ligase" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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