Rat Amyloid Precursor Protein (APP) RTU ELISA Kit | APP rtu elisa kit

Rat Amyloid Precursor Protein (APP) ELISA Kit

Cat. Num. AAA457734

• Gene Names: App; Abeta
• Reactivity: Rat
• Synonyms: Amyloid Precursor Protein (APP); Rat Amyloid Precursor Protein (APP) ELISA Kit; AAA; ABeta; ABPP; AD1; APPI; CVAP; PN2; CTFgamma; Peptidase Nexin-II Alzheimer Disease; Peptidase Nexin-II; Amyloid Beta(A4)Precursor Protein; APP rtu elisa kit

• Reactivity: Rat
• Sequence Length: 770

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 1.25-80ng/mL
• Sensitivity: 0.49ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APP rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of APP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27436861
• NCBI GeneID: 54226
• NCBI Accession #: NP_062161.1
• NCBI GenBank Nucleotide #: NM_019288.2
• UniProt Accession #: P08592; Q547B7
• Molecular Weight: – Da
• NCBI Official Full Name: amyloid-beta A4 protein
• NCBI Official Synonym Full Names: amyloid beta precursor protein
• NCBI Official Symbol: App
• NCBI Official Synonym Symbols: Abeta
• NCBI Protein Information: amyloid-beta A4 protein; amyloid beta A4 protein
• UniProt Protein Name: Amyloid-beta A4 protein
• Protein Family: Amyloid beta A4 protein
• UniProt Gene Name: App
• UniProt Synonym Gene Names: APP; AG; S-APP-alpha; S-APP-beta; Beta-CTF; Abeta42; Abeta40; Alpha-CTF

NCBI Description

activates voltage dependent calcium channels; may induce neuronal apoptosis [RGD, Feb 2006]

Uniprot Description

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions (). Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb (). Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (). May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV (). The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured mitochondrial dysfunction in cultured cortical neurons. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1 ().