Lactate Dehydrogenase B Active Protein | LDHB active protein
Recombinant Lactate Dehydrogenase B
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Lactate Dehydrogenase B; Recombinant Lactate Dehydrogenase B; LDHB; Lactate Dehydrogenase B Recombinant; Lactate Dehydrogenase; LDH; LDHB active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Sterile lyophilized powder.
Sterile lyophilized powder.
Sequence Length
134
Unit Defenition
One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25 degree C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.
Solubility
It is recommended to reconstitute the lyophilized LDH in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The specific activity was found to be 258 U/mg protein.
Preparation and Storage
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution LDH should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Related Product Information for LDHB active protein
Description: The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.
Introduction: Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Introduction: Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Product Categories/Family for LDHB active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
34,192 Da
NCBI Official Full Name
ldh, partial
UniProt Protein Name
L-lactate dehydrogenase 1
Protein Family
UniProt Gene Name
ldh1
UniProt Synonym Gene Names
ldh; L-LDH 1
UniProt Entry Name
LDH1_LISMO
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Product Notes
The LDHB ldh1 (Catalog #AAA142420) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lactate Dehydrogenase B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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