LDH-B (L-lactate dehydrogenase B chain) Polyclonal Antibody | anti-LDH-B antibody

LDH-B (L-lactate dehydrogenase B chain) Antibody

Cat. Num. AAA857605

• Gene Names: LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: LDH-B (L-lactate dehydrogenase B chain); Polyclonal Antibody; LDH-B (L-lactate dehydrogenase B chain) Antibody; anti-LDH-B antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of LDH-B.
• Purity/Purification: Affinity purified
• Form/Format: 1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
• Sequence Length: 334

• Applicable Applications for anti-LDH-B antibody: Western Blot (WB)
• Application Notes: WB: 1:5,000
• Preservative: 0.05% Sodium Azide.
• Cellular Localization: Cytoplasm
• Positive Control: Hela/Mouse Brain
• Preparation and Storage: Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for anti-LDH-B antibody

Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.

References

1. Takeno T., Li S.S.-L.; "Structure of the human lactate dehydrogenase B gene."; Biochem. J. 2.7:92.92.(1.89). 2. The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 1.:2.1.2.7(2.04). 3. Sakai I., Sharief F.S., Pan Y.-C.E., Li S.S.-L.; "The cDNA and protein sequences of human lactate dehydrogenase B."; Biochem. J. 2.8:93.-93.(1.87).

NCBI and Uniprot Product Information

• NCBI GI #: 291575128
• NCBI GeneID: 3945
• NCBI Accession #: NP_001167568.1
• NCBI GenBank Nucleotide #: NM_001174097.2
• UniProt Accession #: P07195
• Molecular Weight: 37kDa
• NCBI Official Full Name: L-lactate dehydrogenase B chain isoform LDHB
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: LDHB
• NCBI Official Synonym Symbols: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• UniProt Gene Name: LDHB
• UniProt Synonym Gene Names: LDH-B; LDH-H

NCBI Description

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]