Mouse anti-Human CBL Monoclonal Antibody | anti-CBL antibody

CBL Antibody

Cat. Num. AAA9217325

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: CBL; Monoclonal Antibody; CBL Antibody; E3 ubiquitin-protein ligase CBL; 6.3.2.-; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL; CBL2; RNF55; Purified Mouse Monoclonal Antibody (Mab); anti-CBL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Specificity: This CBL antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 450-644 amino acids from human CBL.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: 0.98 (varies by lot)
• Sequence Length: 906

• Applicable Applications for anti-CBL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Cellular Location: Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-CBL Antibody at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: Jurkat whole cell lysateLane 3: Raji whole cell lysateLane 4: SK-BR-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CBL antibody

Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

Product Categories/Family for anti-CBL antibody

Crown Antibodies; Cancer; Cell Biology; Signal Transduction

References

Blake T.J.,et al.Oncogene 6:653-657(1991). Taylor T.D.,et al.Nature 440:497-500(2006). Howlett C.J.,et al.Biochem. Biophys. Res. Commun. 257:129-138(1999). Joazeiro C.A.,et al.Science 286:309-312(1999). Donovan J.A.,et al.J. Biol. Chem. 269:22921-22924(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 251757253
• NCBI GeneID: 867
• UniProt Accession #: P22681; A3KMP8
• Molecular Weight: 99633 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL
• NCBI Official Synonym Full Names: Cbl proto-oncogene
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55; FRA11B
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• Protein Family: CBL-interacting protein kinase
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55
• UniProt Entry Name: CBL_HUMAN

NCBI Description

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]

Uniprot Description

CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.19; Oncoprotein; EC 6.3.2.-; Adaptor/scaffold; Ubiquitin conjugating system; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; cytosol; flotillin complex; plasma membrane

Molecular Function: ephrin receptor binding; protein binding; SH3 domain binding; transcription factor activity; ubiquitin-protein ligase activity

Biological Process: cell surface receptor linked signal transduction; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of receptor-mediated endocytosis; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; transforming growth factor beta receptor signaling pathway

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia