Interleukin-21 Active Protein | IL 21 active protein

Recombinant Human Interleukin-21

Cat. Num. AAA142284

• Gene Names: IL21; Za11; IL-21; CVID11
• Purity: Greater than 97.0% as determined by SDS-PAGE.
• Synonyms: Interleukin-21; Recombinant Human Interleukin-21; Za11; IL-21; IL 21 active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by SDS-PAGE.
• Form/Format: Lyophilized from PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
• Sequence Length: 153

• Solubility: It is recommended to reconstitute the lyophilized Interleukin -21 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Measured by its ability to proliferate activated B cells.
• Preparation and Storage: Lyophilized Interleukin-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL21 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for IL 21 active protein

Description: IL21 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a total molecular mass of 15.4kDa. The IL-21 is purified by proprietary chromatographic techniques.

Introduction: IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (degree C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.

Product Categories/Family for IL 21 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 333033767
• NCBI GeneID: 59067
• NCBI Accession #: NP_001193935.1
• NCBI GenBank Nucleotide #: NM_001207006.2
• UniProt Accession #: Q9HBE4; A5J0L4
• Molecular Weight: 16,867 Da
• NCBI Official Full Name: interleukin-21 isoform 2
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: IL21
• NCBI Official Synonym Symbols: Za11; IL-21; CVID11
• NCBI Protein Information: interleukin-21; interleukin-21 isoform
• UniProt Protein Name: Interleukin-21
• Protein Family: Interleukin
• UniProt Gene Name: IL21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_HUMAN

NCBI Description

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; positive regulation of natural killer cell mediated cytotoxicity; signal transduction; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tissue remodeling; positive regulation of cell proliferation; positive regulation of B cell proliferation; immune response; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response

Disease: Immunodeficiency, Common Variable, 11