Human IL-21 ELISA Kit | IL21 elisa kit

Human IL-21 ELISA Kit

Cat. Num. AAA8244689

• Gene Names: IL21; Za11; IL-21; CVID11
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-21; Human IL-21 ELISA Kit; Interleukin-21; Za11; IL21 elisa kit

• Reactivity: Human
• Specificity: The Human IL-21 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-21 proteins within the range of 31.3 pg/ml - 2000 pg/ml.
• Sequence Length: 155

• Applicable Applications for IL21 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for IL21 elisa kit

Principle of the Assay||The Human IL-21 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-21 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in native B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL21/IL21R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

NCBI and Uniprot Product Information

• NCBI GI #: 55976599
• NCBI GeneID: 59067
• UniProt Accession #: Q9HBE4; A5J0L4
• Molecular Weight: 16,867 Da
• NCBI Official Full Name: Interleukin-21
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: IL21
• NCBI Official Synonym Symbols: Za11; IL-21; CVID11
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• Protein Family: Interleukin
• UniProt Gene Name: IL21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_HUMAN

NCBI Description

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Apoptosis; Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space; intracellular

Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding

Biological Process: cell maturation; immune response; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of cytokine production; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-10 production; positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; positive regulation of natural killer cell differentiation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of T cell proliferation; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; signal transduction; tyrosine phosphorylation of Stat3 protein

Disease: Immunodeficiency, Common Variable, 11