Rabbit anti-Human, Mouse WIBG Polyclonal Antibody | anti-WIBG antibody

WIBG, NT (WIBG, PYM, Partner of Y14 and mago, Protein wibg homolog) (Biotin)

Cat. Num. AAA6363601

• Gene Names: PYM1; PYM; WIBG
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WIBG; Polyclonal Antibody; NT (WIBG; PYM; Partner of Y14 and mago; Protein wibg homolog) (Biotin); Protein wibg homolog; anti-WIBG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-WIBG antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human WIBG.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WIBG Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the WIBG antibody detected the WIBG protein (arrow).)

Immunohistochemistry (IHC)

(WIBG antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIBG antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-WIBG antibody

Antibodies; Transcription Factors

References

Gehring, N.H., et al. Cell 137(3):536-548(2009). Diem, M.D., et al. Nat. Struct. Mol. Biol. 14(12):1173-1179(2007). Forler, D., et al. Nat. Biotechnol. 21(1):89-92(2003). Gatfield, D., et al. J. Cell Biol. 159(4):579-588(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 14150139
• NCBI GeneID: 84305
• NCBI Accession #: NP_115721.1
• NCBI GenBank Nucleotide #: NM_032345.2
• UniProt Accession #: Q9BRP8
• NCBI Official Full Name: partner of Y14 and mago isoform 1
• NCBI Official Synonym Full Names: PYM homolog 1, exon junction complex associated factor
• NCBI Official Symbol: PYM1
• NCBI Official Synonym Symbols: PYM; WIBG
• NCBI Protein Information: partner of Y14 and mago
• UniProt Protein Name: Partner of Y14 and mago
• UniProt Gene Name: WIBG
• UniProt Synonym Gene Names: PYM
• UniProt Entry Name: WIBG_HUMAN

Uniprot Description

WIBG: a conserved protein of unknown function. A potential RNA-binding protein.

Protein type: RNA-binding; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus

Molecular Function: protein binding; ribosome binding

Biological Process: positive regulation of translation; mRNA catabolic process, nonsense-mediated decay