Canine Interleukin 21, IL-21 ELISA Kit | IL-21 elisa kit

Canine Interleukin 21, IL-21 ELISA Kit

Cat. Num. AAA166438

• Gene Names: IL21; Za11; IL-21; CVID11
• Reactivity: Canine
• Synonyms: Interleukin 21; IL-21; Canine Interleukin 21; IL-21 ELISA Kit; IL-21 elisa kit

• Reactivity: Canine
• Sequence Length: 153

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 2ng/L - 600ng/L
• Sensitivity: 1.02ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-21 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Interleukin 21 (also known as IL-21) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine IL-21 antibody. IL-21 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine IL-21 Antibody is added and binds to IL-21 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-21 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine IL-21. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 333033767
• NCBI GeneID: 59067
• NCBI Accession #: NP_001193935.1
• NCBI GenBank Nucleotide #: NM_001207006.2
• UniProt Accession #: Q9HBE4; A5J0L4
• Molecular Weight: 16,867 Da
• NCBI Official Full Name: interleukin-21 isoform 2
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: IL21
• NCBI Official Synonym Symbols: Za11; IL-21; CVID11
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• UniProt Gene Name: IL21
• UniProt Synonym Gene Names: IL-21

NCBI Description

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells (). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.