Hexokinase 4 Active Protein | GCK active protein

Recombinant Hexokinase 4

Cat. Num. AAA201110

• Gene Names: GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Hexokinase 4; Recombinant Hexokinase 4; Hexokinase type IV; Glucokinase; GCK; GCK active protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; In 20 mM Tris-HCl Buffer (pH 8.0) Containing 10% Glycerol.
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-465aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ
• Sequence Length: 465

• Applicable Applications for GCK active protein: Enzyme Activity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Biological Activity: Specific activity is > 2,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for GCK active protein

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes -types 1 to 4 hexokinases- have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase 4 is commonly called glucokinase. Recombinant human Hexokinase 4, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GCK active protein

Signal Transduction; Metabolism; Cancer

References

Jon E. et al.,(2003) J.Exp Biology. 206: 2049-2057

NCBI and Uniprot Product Information

• NCBI GI #: 547696
• NCBI GeneID: 2645
• UniProt Accession #: P35557
• Molecular Weight: 54.3kDa (485aa)
• NCBI Official Full Name: Hexokinase-4
• NCBI Official Synonym Full Names: glucokinase
• NCBI Official Symbol: GCK
• NCBI Official Synonym Symbols: GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• NCBI Protein Information: hexokinase-4; glucokinase
• UniProt Protein Name: Glucokinase
• Protein Family: Glycerate 2-kinase
• UniProt Gene Name: GCK
• UniProt Synonym Gene Names: HK IV; HK4
• UniProt Entry Name: HXK4_HUMAN

NCBI Description

This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]