Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Vesicles, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite.)
anti-Human, rat GDNF Polyclonal Antibody | anti-GDNF antibody
Rabbit Anti-Human GDNF Polyclonal Antibody
Reactivity
Human, rat
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
GDNF; Polyclonal Antibody; Rabbit Anti-Human GDNF Polyclonal Antibody; anti-GDNF antibody
Reactivity
Human, rat
Clonality
Polyclonal
Specificity
Predicted molecular weight at ~ 24 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GDNF antibody
Western Blot (WB)
Application Notes
WB (1:1000)
Immunogen
Synthetic peptide from the mid-protein of Human GDNF.
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of GDNF in 15 ug of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization
Secreted
Preparation and Storage
Store at -20 degree C; 1 year+. Avoid freeze/ thaw cycle.
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Vesicles, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Vesicles, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite.)
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~23.7 kDa GDNF protein using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer.)
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~23.7 kDa GDNF protein using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer.)
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The GDNF (Catalog #AAA807916) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human GDNF Polyclonal Antibody reacts with Human, rat and may cross-react with other species as described in the data sheet. AAA Biotech's GDNF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB (1:1000). Researchers should empirically determine the suitability of the GDNF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDNF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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