glial derived neorotropic factor Active Protein | hGDNF active protein

Human glial derived neorotropic factor (hGDNF)

Cat. Num. AAA400009

• Gene Names: GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Synonyms: glial derived neorotropic factor; Human glial derived neorotropic factor (hGDNF); hGDNF active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
• Sequence Length: 202

• Solubility: It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions containing carrier proteins such as BSA or HSA.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of bPL
• Formulation: GDNF was lyophilized after dialysis against 10 mM sodium citrate and 150mM NaCl.
• Biological Activity: The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml.
• Preparation and Storage: Lyophilized Glial Derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GDNF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please avoid freeze-thaw cycles.

Related Product Information for hGDNF active protein

GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Glial Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
GDNF is purified by proprietary chromatographic techniques.

Product Categories/Family for hGDNF active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 299473781
• NCBI GeneID: 2668
• NCBI Accession #: NP_001177398.1
• NCBI GenBank Nucleotide #: NM_001190469.1
• UniProt Accession #: P39905; O95448; O95449; O95986; Q6FH33; Q96L44; Q9UD32; Q9UD33; Q9UMV2; Q9UP67; Q9UP97; B7WPK7; AAB33493; AAB33494
• Molecular Weight: 18,123 Da
• NCBI Official Full Name: glial cell line-derived neurotrophic factor isoform 4 preproprotein
• NCBI Official Synonym Full Names: glial cell derived neurotrophic factor
• NCBI Official Symbol: GDNF
• NCBI Official Synonym Symbols: ATF1; ATF2; HSCR3; HFB1-GDNF
• NCBI Protein Information: glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor
• UniProt Protein Name: Glial cell line-derived neurotrophic factor
• UniProt Gene Name: GDNF
• UniProt Synonym Gene Names: hGDNF; ATF
• UniProt Entry Name: GDNF_HUMAN

NCBI Description

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

Uniprot Description

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; receptor binding

Biological Process: positive regulation of dopamine secretion; axon guidance; nervous system development; peristalsis; adult locomotory behavior; mRNA stabilization; positive regulation of monooxygenase activity; regulation of dopamine uptake; signal transduction; enteric nervous system development; sympathetic nervous system development; regulation of gene expression; ureteric bud branching; induction of an organ; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; negative regulation of neuron apoptosis; postsynaptic membrane organization; positive regulation of cell differentiation; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis

Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 3; Pheochromocytoma