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Western Blot (WB) (All lanes : Anti-Cyclophilin D Antibody at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: CCRF-CEM whole cell lysateLane 5: human brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit Cyclophilin D Polyclonal Antibody | anti-PPID antibody

Cyclophilin D Antibody

Gene Names
PPID; CYPD; CYP-40
Reactivity
Human; Predicted Reactivity: Mouse, Rat
Applications
Western Blot, ELISA
Synonyms
Cyclophilin D; Polyclonal Antibody; Cyclophilin D Antibody; Peptidyl-prolyl cis-trans isomerase D; PPIase D; 5.2.1.8; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D; PPID; CYP40; CYPD; anti-PPID antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Mouse, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This Cyclophilin D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 336-370 amino acids from the human region of human Cyclophilin D.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
370
Applicable Applications for anti-PPID antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm.
Tissue Location
Widely expressed.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Cyclophilin D Antibody at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: CCRF-CEM whole cell lysateLane 5: human brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-Cyclophilin D Antibody at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: CCRF-CEM whole cell lysateLane 5: human brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PPID antibody
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA- binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large- cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.
Product Categories/Family for anti-PPID antibody
References
Kieffer L.J.,et al.J. Biol. Chem. 268:12303-12310 (1993). Yokoi H.,et al.Genomics 35:448-455 (1996). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Gevaert K.,et al.Nat. Biotechnol. 21:566-569 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40764 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase D
NCBI Official Synonym Full Names
peptidylprolyl isomerase D
NCBI Official Symbol
PPID
NCBI Official Synonym Symbols
CYPD; CYP-40
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase D
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase D
UniProt Gene Name
PPID
UniProt Synonym Gene Names
CYP40; CYPD; PPIase D; CYP-40
UniProt Entry Name
PPID_HUMAN

NCBI Description

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]

Uniprot Description

PPID: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.

Protein type: Isomerase; EC 5.2.1.8; Nucleolus

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus

Molecular Function: cyclosporin A binding; estrogen receptor binding; heat shock protein binding; Hsp70 protein binding; Hsp90 protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; transcription factor binding

Biological Process: apoptosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of protein secretion; positive regulation of viral genome replication; protein complex assembly; protein folding; protein peptidyl-prolyl isomerization; protein transport; release of virus from host

Research Articles on PPID

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Product Notes

The PPID ppid (Catalog #AAA9217262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyclophilin D Antibody reacts with Human; Predicted Reactivity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclophilin D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the PPID ppid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclophilin D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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