Glutathione S-Transferase Alpha 4 Active Protein | GSTA4 active protein

Recombinant Human Glutathione S-Transferase Alpha 4, Active

Cat. Num. AAA146843

• Gene Names: GSTA4; GTA4; GSTA4-4
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Glutathione S-Transferase Alpha 4; Recombinant Human Glutathione S-Transferase Alpha 4; Active; GSTA4 Human; Glutathione S-transferase A4; GST class-alpha member 4; Glutathione S-transferase A4-4; GSTA4; GSTA4-4; Glutathione S-Transferase Alpha 4 Human Recombinant; GSTA4 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The GSTA4 solution (1mg/ml) contains 20mM Tris-HCl buffer, pH8.0, 20% glycerol, 2mM DTT and 100mM NaCl.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQDGNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDDQQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTVKLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP
• Sequence Length: 222

• Physical Appearance: Sterile filtered colorless solution.
• Biological Activity: Specific activity is > 4, 000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2, 4- dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for GSTA4 active protein

Description: GSTA4 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (1-222) and having a molecular mass of 28.3kDa.GSTA4 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: Glutathione S-transferase A4 (GSTA4) is a member of the GST superfamily. The GSTA4 enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows an especially high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is extremely effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, which is an important product of peroxidative degradation of arachidonic acid and a frequently used biomarker for oxidative damage in tissue. The GSTA4 enzyme is expressed at a high level in the brain, placenta, and skeletal muscle and much lower in the lung and liver.

Product Categories/Family for GSTA4 active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4504173
• NCBI GeneID: 2941
• NCBI Accession #: NP_001503.1
• NCBI GenBank Nucleotide #: NM_001512.3
• UniProt Accession #: O15217; Q5T7Q8; Q6P4G1; Q9BX18; Q9H414; B2RD15
• NCBI Official Full Name: glutathione S-transferase A4
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 4
• NCBI Official Symbol: GSTA4
• NCBI Official Synonym Symbols: GTA4; GSTA4-4
• NCBI Protein Information: glutathione S-transferase A4
• UniProt Protein Name: Glutathione S-transferase A4
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA4

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTA4: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4- hydroxynonenal (4-HNE). Belongs to the GST superfamily. Alpha family.

Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 6p12.2

Cellular Component: cytosol

Molecular Function: glutathione transferase activity; identical protein binding; protein binding; protein homodimerization activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process