Glutathione S-Transferase Alpha 1 Active Protein | GSTA1 active protein

Recombinant Human Glutathione S-Transferase Alpha 1

Cat. Num. AAA143651

• Gene Names: GSTA1; GST2; GTH1; GSTA1-1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Glutathione S-Transferase Alpha 1; Recombinant Human Glutathione S-Transferase Alpha 1; GSTA1 Human; Glutathione S-Transferase Alpha-1 Human Recombinant; GST2; GSTA1-1; GTH1; GSTA-1; GSTAI; GSTA-I; EC 2.5.1.18; Glutathione S-transferase A1; GST HA subunit 1; GST-epsilon; GST class-alpha member 1; GSTA1; MGC131939; GSTA1 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The GSTA1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPLLKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
• Sequence Length: 222

• Biological Activity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 degree C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for GSTA1 active protein

Description: GSTA1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 222 amino acids (1-222 a.a.) and having a molecular mass of 25.6 kDa. The GSTA1 is purified by proprietary chromatographic techniques.

Introduction: Membrane-bound & Cytosolic forms of GST are encoded by 2 separate supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. There are 8 different classes of soluble cytoplasmic mammalian GST: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTA1 is found in a cluster mapped to chromosome 6, and is highly expressed in the liver. GSTA1 protects the cells from reactive oxygen species.

Product Categories/Family for GSTA1 active protein

Enzymes; Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 22091454
• NCBI GeneID: 2938
• NCBI Accession #: NP_665683.1
• NCBI GenBank Nucleotide #: NM_145740.3
• UniProt Accession #: P08263; Q14750; Q5GHF8; Q5SZC1
• Molecular Weight: 25,631 Da
• NCBI Official Full Name: glutathione S-transferase A1
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 1
• NCBI Official Symbol: GSTA1
• NCBI Official Synonym Symbols: GST2; GTH1; GSTA1-1
• NCBI Protein Information: glutathione S-transferase A1; GST HA subunit 1; GST class-alpha member 1; GST, class alpha, 1; GST-epsilon; S-(hydroxyalkyl)glutathione lyase A1; glutathione S-alkyltransferase A1; glutathione S-aryltransferase A1; glutathione S-transferase 2; glutathione S-transferase Ha subunit 1
• UniProt Protein Name: Glutathione S-transferase A1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA1
• UniProt Entry Name: GSTA1_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTA1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family.

Protein type: Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol

Molecular Function: glutathione transferase activity

Biological Process: glutathione metabolic process; epithelial cell differentiation; metabolic process; xenobiotic metabolic process