Importin 8 Recombinant Protein | IPO8 recombinant protein

Recombinant Importin 8 (IPO8)

Cat. Num. AAA2030782

• Gene Names: IPO8; RANBP8
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Importin 8; Recombinant Importin 8 (IPO8); IPO8 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.9 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-YQIL TDPNFDPRKK DGALHVIGSL AEILLKKSLF KDQIELFLQN HVFPLLMSNL GYLRARSCWV LHAFSSLKFH NELNLRNAVE LAKKSLIEDE EMPVKVEAAL ALQSLISNQA QAKEHMKPYV RFIMQELLHI VRETENDDVT NVIQKLICEY SQDVASIAVD TTQHLAEIFG KVLQSDEYEE IEDKTVMAMGILHTIDTILT VV
• Sequence Length: 1037

• Applicable Applications for IPO8 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 55.9kDa
• Accurate Molecular Mass (KD): 54kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Tyr417~Val622
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 53759103
• NCBI GeneID: 10526
• NCBI Accession #: NP_006381.2
• NCBI GenBank Nucleotide #: NM_006390.3
• Molecular Weight: 96,036 Da
• NCBI Official Full Name: importin-8 isoform 1
• NCBI Official Synonym Full Names: importin 8
• NCBI Official Symbol: IPO8
• NCBI Official Synonym Symbols: RANBP8
• NCBI Protein Information: importin-8
• UniProt Protein Name: Importin-8
• Protein Family: Importin
• UniProt Gene Name: IPO8
• UniProt Synonym Gene Names: RANBP8; Imp8; RanBP8
• UniProt Entry Name: IPO8_HUMAN

NCBI Description

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

IPO8: Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter- like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear export; Karyopherin; G protein regulator, misc.; Nuclear import

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; Ran GTPase binding

Biological Process: intracellular protein transport; gene expression; signal transduction