Klenow Fragment 3'-->5' exo DNA Manipulation

Klenow Fragment 3'-->5' exo

Cat. Num. AAA9141654

• Applications: A-tailing, Polymerases for DNA Manipulation, DNA Sequencing, RT-PCR & cDNA Synthesis, PCR
• Synonyms: Klenow Fragment 3'-->5' exo; Klenow Fragment 3'-->5' exo dna manipulation

• Host: E Coli
• Form/Format: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25 degree C
• Concentration: 5,000 U/ml (varies by lot)

• Applicable Applications for Klenow Fragment 3'-->5' exo dna manipulation: A-tailing, Polymerases for DNA Manipulation, DNA Sequencing, RT-PCR & cDNA Synthesis, PCR
• Source Note: An E Coli strain containing a plasmid with a fragment of the E Coli polA (D355A, E357A) gene starting at codon 324.
• Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37 degree C.
• Reaction Conditions: 1x ABuffer B, Incubate at 37 degree C
• Reaction Buffer or Mix: 1X ABuffer B:; 10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 at 25 degree C
• Heat Inactivation: 75 degree C for 20 min
• 5' - 3' Exonuclease: No
• 3' - 5' Exonuclease: No
• Strand Displacement: +++
• Error Rate: ~ 100 x 10^6 bases
• Preparation and Storage: Store at -20 degree C.

Related Product Information for Klenow Fragment 3'-->5' exo dna manipulation

Klenow Fragment (3'--> 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5'--> 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3'--> 5' exonuclease activity. Klenow Fragment (3'--> 5' exo-) is isolated from a recombinant source. It generates probes using random primers and shows moderate strand displacement activity.
It is applicable to random priming labeling, DNA sequencing by the Sanger dideoxy method, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.