CCL7 active protein

Human CCL7 (MCP-3)

Cat. Num. AAA444005

• Gene Names: CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• Purity: > 97% by SDS PAGE
• Synonyms: CCL7; Human CCL7 (MCP-3); CCL7 (MCP-3); CCL7 active protein

• Host: E Coli derived
• Purity/Purification: > 97% by SDS PAGE
• Form/Format: Lyophilized
• Sequence Positions: 24-99
• Sequence: QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWV QDFMKHLDKKTQTPKL
• Sequence Length: 99

• Reconstitution: Spin sample prior to reconstitution. Recommended at 100mug/mL in sterile water
• Carrier Protein: None
• Activity: EC50 = 0.44 nM determined by migration assay with cells expressing recombinant CCR2
• Extinction Coefficient: 8,730 M-1 cm-1
• Endotoxin Level: <0.01 EU per 1mug of the protein by the LAL method
• Modification: None
• UNSPSC Code: 12352202
• Preparation and Storage: Spin sample prior to reconstitution. Avoid repeated freeze-thaw cycles; 12 months from date of receipt, -20 to -70 degree C as supplied.; 1 month, 2 to 8 degree C under sterile conditions after reconstitution.; 3 months, -20 to -70 degree C under sterile conditions after reconstitution.

Testing Data

Related Product Information for CCL7 active protein

Monocyte chemotactic protein 3 (MCP-3/CCL7) is secreted by monocytes and certain tumor cell lines. It is a chemoattractant for monocytes and other leukocytes. MCP-3 binds and signals through several chemokine receptors including CCR1, CCR2, CCR3, and appears to be an antagonist for CCR5.

References

1. "Monocyte chemotactic protein-3" Menten P., Wuyts A., Van Damme J. Eur. Cytokine Netw. 12:554-560 (2002)
2. "Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines". Opdenakker G., Froyen G., Fiten P., Proost P., Van Damme J. Biochem. Biophys. Res. Commun. 191: 535-42 (1993)
3. "CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist." Blanpain C., Migeotte I., Lee B., Vakili J., Doranz B.J., Govaerts C., Vassart G., Doms R.W., Parmentier M.Blood 94:1899-1905 (1999)

NCBI and Uniprot Product Information

• NCBI GI #: 13435402
• NCBI GeneID: 6354
• NCBI Accession #: NP_006264.2
• NCBI GenBank Nucleotide #: NM_006273.3
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: Actual Molecular Mass: Mass spec data confirmed the expected mass of 8.956 kDa; Predicted Molecular Mass: 8.956 kDa
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 7
• NCBI Official Symbol: CCL7
• NCBI Official Synonym Symbols: FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• NCBI Protein Information: C-C motif chemokine 7; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3); small-inducible cytokine A7
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

NCBI Description

This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; cytoskeleton organization and biogenesis; immune response; inflammatory response; signal transduction; chemotaxis; positive regulation of cell migration