Western Blot (WB)
(Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.)
Rabbit anti-Human, Mouse Smoothened Polyclonal Antibody | anti-SMO antibody
Anti-Smoothened Antibody
Western Blot (WB)
(Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
Uniprot Description
SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.
Protein type: Oncoprotein; Membrane protein, multi-pass; Membrane protein, integral; GPCR, Fz/Smo family; Receptor, GPCR; Cell development/differentiation
Chromosomal Location of Human Ortholog: 7q32.3
Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; integral to membrane; plasma membrane; caveola
Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; patched binding; drug binding
Biological Process: developmental growth; central nervous system neuron differentiation; ventral midline determination; skeletal muscle fiber development; negative regulation of epithelial cell differentiation; cell fate specification; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; thalamus development; anterior/posterior pattern formation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; heart looping; vasculogenesis; neural crest cell migration; activation of hh target transcription factor; dorsoventral neural tube patterning; positive regulation of neuroblast proliferation; smoothened signaling pathway; hair follicle morphogenesis; protein stabilization; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; in utero embryonic development; multicellular organism growth; smoothened signaling pathway in ventral spinal cord patterning; forebrain morphogenesis; negative regulation of DNA binding; negative regulation of hair follicle development; odontogenesis of dentine-containing teeth; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein import into nucleus; cerebellar cortex morphogenesis; myoblast migration; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Research Articles on SMO
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Product Notes
The SMO smo (Catalog #AAA8239889) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Smoothened Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Smoothened can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the SMO smo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smoothened, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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