Mouse anti-Human SOD2 Monoclonal Antibody | anti-SOD2 antibody

SOD2 Antibody

Cat. Num. AAA9200521

• Gene Names: SOD2; IPOB; MNSOD; MVCD6
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: SOD2; Monoclonal Antibody; SOD2 Antibody; Superoxide dismutase [Mn]; mitochondrial; anti-SOD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1κ
• Clone Number: 37CT127.5.11.6
• Specificity: Purified His-tagged SOD protein(Fragment) was used to produced this monoclonal antibody.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.34 (varies by lot)
• Sequence Length: 222

• Applicable Applications for anti-SOD2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:2000; IHC~~1:10~50
• Antigen Type: Recombinant Protein
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-SD Antibody at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: human brain lysatesLane 3: human heart lysatesLane 4: human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOD2 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-SOD2 Monoclonal Antibody by SOD2-GST fusion protein (GST MW=26 kD. SOD2-GST fusion protein (arrow) was detected using the purified Mab. (1:2000))

Related Product Information for anti-SOD2 antibody

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-SOD2 antibody

Crown Antibodies; Cancer; Cardiovascular; Cell Biology; Metabolism; Neuroscience; Signal Transduction

References

The sequence polymorphism of MnSOD gene in subjects with respiratory insufficiency in COPD. Pietras T, et al. Med Sci Monit, 2010 Aug 7. PMID 20802415.
Association between the rs4880 superoxide dismutase 2 (C>T) gene variant and coronary heart disease in diabetes mellitus. Jones DA, et al. Diabetes Res Clin Pract, 2010 Aug 20. PMID 20728955.
Assessing oxidative pathway genes as risk factors for bipolar disorder. Fullerton JM, et al. Bipolar Disord, 2010 Aug. PMID 20712757.
Do genetic variations in antioxidant enzymes influence the course of hereditary hemochromatosis? Nahon P, et al. Antioxid Redox Signal, 2010 Aug 1. PMID 20673159.
Manganese superoxide dismutase versus p53: the mitochondrial center. Holley AK, et al. Ann N Y Acad Sci, 2010 Jul. PMID 20649542.

NCBI and Uniprot Product Information

• NCBI GI #: 67782305
• NCBI GeneID: 6648
• NCBI Accession #: NP_000627.2; NP_001019636.1; NP_001019637.1
• NCBI GenBank Nucleotide #: NM_000636.2
• UniProt Accession #: P04179; P78434; Q16792; Q5TCM1; Q96EE6; Q9P2Z3; B2R7R1; B3KUK2; B4DL20; B4E3K9; E1P5A9
• Molecular Weight: 24722
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 2, mitochondrial
• NCBI Official Symbol: SOD2
• NCBI Official Synonym Symbols: IPOB; MNSOD; MVCD6
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• Protein Family: Superoxide dismutase
• UniProt Gene Name: SOD2
• UniProt Entry Name: SODM_HUMAN

NCBI Description

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.15.1.1; Mitochondrial

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: identical protein binding; DNA binding; manganese ion binding; superoxide dismutase activity; oxygen binding

Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; response to lipopolysaccharide; locomotory behavior; response to L-ascorbic acid; post-embryonic development; protein homotetramerization; negative regulation of cell proliferation; response to selenium ion; glutathione metabolic process; regulation of mitochondrial membrane potential; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; response to axon injury; negative regulation of neuron apoptosis; response to electrical stimulus; response to drug; erythrophore differentiation; response to superoxide; release of cytochrome c from mitochondria; superoxide metabolic process; liver development; negative regulation of fat cell differentiation; regulation of transcription from RNA polymerase II promoter; response to manganese ion; iron ion homeostasis; response to reactive oxygen species; response to silicon dioxide; response to hyperoxia; response to cadmium ion; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to zinc ion; age-dependent response to reactive oxygen species; negative regulation of fibroblast proliferation; detection of oxygen; response to hypoxia; neuron development; response to cold; response to activity; induction of apoptosis by oxidative stress; superoxide release; hydrogen peroxide biosynthetic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6